Human FGF R3 MAb (Clone 136318) (25 UG)【科瑞奥博】

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Human FGF R3 MAb (Clone 136318) (25 UG)

货号： MAB7661-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB7661-SP
别名	CD333; CEK; EC 2.7.10; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism
全称	Fibroblast Growth Factor Receptor 3
反应种属	Human
应用	Western Blot,Neutralization
目标/特异性	Detects the IIIb and IIIc isoforms of human FGF R3. It does not cross-react with mouse FGF R3 or with any isoforms of recombinant human (rh) FGF R1 or rhFGF R2.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109: 749.The Neutralization Dose (ND₅₀) is typically 2.5-15 ng/mL in the presence of 6 ng/mL Recombinant Human FGF R3 (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
来源	Monoclonal Mouse IgG₁ Clone # 136318
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2261 (Human); 14184 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I Stem Cells, 2012;30(7):1455-64. Species: Human Sample Type: Whole Cells Application: Neut Prox1 promotes lineage-specific expression of fibroblast growth factor (FGF) receptor-3 in lymphatic endothelium: a role for FGF signaling in lymphangiogenesis. Authors: Shin JW, Min M, Larrieu-Lahargue F, Canron X, Kunstfeld R, Nguyen L, Henderson JE, Bikfalvi A, Detmar M, Hong YK Mol. Biol. Cell, 2005;17(2):576-84. Species: Human Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIc)
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The extracellular domain of FGF R3 includes 3 Ig-like domains. Asegment of the membrane proximal Ig-like domain can be encoded by twodifferent exons resulting in (IIIb) or (IIIc) isoforms.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers FGF Family FGF Ligands FGF Regulators Fibroblast Growth Factor Superfamily Fibroblast Growth Factors and their Receptors Glial Lineage Markers Human CD Marker Products ICC / IHC Images: FGF R3 Mini-review: Fibroblast Growth Factors (FGFs) Multiple Myeloma Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway

参考图片

FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R3 Antibody. Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R3 Monoclonal Antibody (Catalog # MAB7661). The ND₅₀ is typically 0.01-0.04 µg/mL in the presence of heparin (10 µg/mL).