| 货号 | MAB543-SP |
| 别名 | LIX |
| 反应种属 | Rat |
| 应用 | Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects rat LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) LIX is observed, 5% cross-reactivity with recombinant rat (rr) CXCL2 is observed, and no cross-reactivity with recombinant human rhCXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, or 13, rmCXCL1, 2, 9, 10, 11, 12, 13, or 15, recombinant porcine (rp) CXCL8, or rrCXCL1 or 3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.2 µg/mL Recombinant Rat LIX. |
| 来源 | Monoclonal Mouse IgG1 Clone # 135803 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 20311 (Mouse); 60665 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant rat LIX Ala38-Arg125, Ala38-Lys117 Accession # P97885.1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-10 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemotactic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Rat LIX is synthesized as a 130 amino acid (aa) precursor that contains a 37 aa signal sequence plus a 93 aa mature region (aa 38-130) that likely possesses a cleavable 13 aa C-terminus (SwissProt #:P97885). The mature region possesses a receptor-binding ELR/GluLeuArg motif between aa 47-50, and an alpha -family characteristic CxC motif between aa 50-52. Although there are no known splice variants of rat LIX, based on mouse, considerable proteolytic processing likely occurs at both the N- and C-termini over aa 38-130. This may reduce the MW in SDS-Page by as much as 3 kDa. The majority of LIX could be expected to start between aa 43-46, and this is likely to be positively correlated with bioactivity. Over aa 38-117, rat LIX shares 73% aa sequence identity with mouse LIX. Although not a strict ortholog, rat LIX also shares 64% aa sequence identity with human GCP-2.
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| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by LIX and Neutralization by Rat LIX Antibody. Recombinant Rat LIX (Catalog # 543-RL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat LIX (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat LIX Monoclonal Antibody (Catalog # MAB543). The ND50 is typically 1-5 µg/mL. |
