Human CXCR5 (BLR-1) MAb (Clone 51505) (25 UG)【科瑞奥博】

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Human CXCR5 (BLR-1) MAb (Clone 51505) (25 UG)

货号： MAB190-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB190-SP
简介	Clone 51505 was used by HLDA to establish CD designation
别名	BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15
反应种属	Human
应用	Flow Cytometry,Immunohistochemistry,CyTOF-reported,Immunocytochemistry,Neutralization
目标/特异性	Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND₅₀) is typically 0.25-1.5 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.
来源	Monoclonal Mouse IgG_2B Clone # 51505
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	643 (Human); 12145 (Mouse); 29363 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Capture-stabilize approach for membrane protein SPR assays. Authors: Chu, Ruiyin, Reczek, David, Brondyk, William Sci Rep, 2014;4(0):7360. Species: Human Sample Type: Peptide (CXCR5 N-terminal) Application: Biacore IL-15 positively regulates IL-21 production in celiac disease mucosa. Authors: Sarra M, Cupi M, Monteleone I, Franze E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi O, Corazza G, Pallone F, Monteleone G Mucosal Immunol, 2013;6(2):244-55. Species: Human Sample Type: Whole Tissue Application: IHC - Frozen Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Authors: Islam SA, Chang DS, Colvin RA Nat. Immunol., 2011;12(2):167-77. Species: Human Sample Type: Whole Cells Application: Flow Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW Cancer Lett., 2009;283(1):29-35. Species: Human Sample Type: Whole Cells Application: Neut Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Authors: Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D Br. J. Cancer, 2008;99(6):930-8. Species: Human Sample Type: Tissue Homogenates Application: WB Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Authors: Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A Blood, 2008;112(12):4401-10. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia. Authors: Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA Blood, 2007;110(9):3316-25. Species: Human Sample Type: Whole Cells Application: Neut The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J Stem Cells, 2007;25(7):1737-45. Species: Human Sample Type: Whole Cells Application: Flow Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH J. Immunol., 2007;179(6):3724-33. Species: Human Sample Type: Whole Cells Application: Flow Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. Authors: Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A J. Cell. Physiol., 2006;207(2):364-73. Species: Human Sample Type: Whole Cells Application: ICC Chemokine receptor expression in rat adjuvant-induced arthritis. Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE Arthritis Rheum., 2005;52(12):3718-30. Species: Rat Sample Type: Whole Tissue Application: IHC Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1. Authors: Sinha RK, Mage RG Dev. Comp. Immunol., 2004;28(7):829-41. Species: Rabbit Sample Type: Whole Cells Application: Flow Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. Authors: Lim HW, Hillsamer P, Kim CH J. Clin. Invest., 2004;114(11):1640-9. Species: Human Sample Type: Whole Cells Application: ICC Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D J. Immunol., 2004;173(11):6592-602. Species: Human Sample Type: Whole Cells Application: Flow Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, RÃƒÂ©vÃƒÂ©sz T Blood, 2003;103(7):2806-8. Species: Human Sample Type: Whole Cells Application: Flow Expression of functional CCR and CXCR chemokine receptors in podocytes. Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H J. Immunol., 2002;168(12):6244-52. Species: Human Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0 mouse myeloma cell line transfected with human CXCR5 Met1-Phe372 Accession # P32302
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCR5, also known as BLR-1, is a 7 transmembrane domain proteinexpressed on B cells. CXCR5 mediates B cell migration following bindingof CXCL13.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion B Cell Markers Chemokine Receptors Chemokines and Receptors in Angiogenesis Chemokines and Receptors in Tfh Cell Differentiation and Function Human CD Marker Products ICC / IHC Images: CXCR5 LTi Cells T Cell Migration/Adhesion

参考图片

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody.
Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose‑dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA‑1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190). The ND₅₀ is typically
0.25-1.5 µg/mL.

CXCR5 in Human PBMCs. CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CXCR5 in Human Kidney. CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.