| 货号 | MAB4169-SP |
| 别名 | alpha HCG; CGA glycoprotein hormones, alpha polypeptide; CGA; CG-ALPHA; FSHA; GPHa; GPHa1; HCG; LHA; TSHA |
| 反应种属 | Human |
| 应用 | Western Blot,Immunohistochemistry |
| 目标/特异性 | Detects human Chorionic Gonadotropin, alpha Chain ( alpha HCG) in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human FSH beta or recombinant rat FSH beta is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1081 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Chorionic Gonadotropin, alpha Chain ( alpha HCG) Ala25-Ser116 Accession # P01215 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human Chorionic Gonadotropin, alpha chain (CGA) is a 92 aa glycopeptide that functions as the shared alpha subunit of the heterodimeric peptide hormones choriogonadotropin, leutinizing hormone, thryoid stimulating hormone, and follicle stimulating hormone. CGA circulates as a free molecule and in noncovalent complexes with the unique beta subunits of those hormones. CGA is secreted by the pituitary and placenta. Mature human CGA shares 69%‑73% aa sequence identity with canine, equine, feline, mouse, porcine, and rat CGA. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chorionic Gonadotropin, alpha Chain ( alpha HCG) in Human Placenta. Chorionic Gonadotropin, alpha Chain ( alpha HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, alpha Chain ( alpha HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
