| 货号 | MAB64892-SP |
| 别名 | ACAN; AGC1; AGC1SEDK; aggrecan core protein; aggrecan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1; CSPG1aggrecan 1; CSPGCP; large aggregating proteoglycan; MSK16; MSK16AGCAN; SEDK |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human Aggrecan in direct ELISAs. It specifically recognizes the N-terminal fragment of recombinant human Aggrecan released following cleavage by ADAMTS5. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 176 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | KLHG-conjugated human Aggrecan synthetic peptide Leu384-Glu392 Accession # P16112 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Aggrecan, together with type II collagen, makes up to 90% of the dry weight of healthy cartilage. It hydrates the collagen network and thus provides cartilage with its properties of compressibility and elasticity. The N-terminal region of human Aggrecan (residues 20‑675) consists of two globular domains (G1 and G2), flanking an interglobular domain (IGD). The IGD contains the cleavage sites for matrix metalloproteinases and aggrecanases. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot. Western blot shows recombinant human Aggregan N-terminal and C-terminal fragments. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aggrecan Monoclonal Antibody (Catalog # MAB64892) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the Aggrecan N-terminal fragment at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
