| 货号 | MAB9502-SP |
| 别名 | EC 1.14.13.39; Hepatocyte NOS; HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2; Inducible NO synthase; Inducible NOS; INOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS; NOS, type II; NOS2; NOS2A; NOS2ANOS type II | 全称 | Inducible Nitic Oxide Synthase |
| 反应种属 | Human |
| 应用 | Western Blot,Simple Western,Immunohistochemistry |
| 目标/特异性 | Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4843 (Human); 18126 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Recombinant human iNOS Pro781-His798 Accession # P35228 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Inducible nitric oxide synthase (iNOS)is a widely expressed, approximately 130 kDa enzyme that catalyzes theconversion of L-arginine to nitric oxide (NO) plus citrulline. NO is abioactive mediator that plays an important role in hemodynamics by inducingvascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney.In other tissues, it is inducibly expressed during inflammation, oxidativestress, and hyperglycemia. iNOS activity is elevated in a variety of diseasestates including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acutekidney injury, TNF-alpha induced muscle wasting, and the increasedradiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOSshares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human iNOS aa 781-798 by Western Blot. Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # 201‑LB,210‑TA,285‑IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human iNOS Monoclonal Antibody (Catalog # MAB9502), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6. | |
iNOS in Human Brain. iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human iNOS aa 781-798 Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
