| 货号 | MAB8336-SP |
| 别名 | avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 | 全称 | Epidermal Growth Factor Receptor/Receptor Tyrosine Protein Kinase ErbB1 |
| 反应种属 | Human |
| 应用 | Western Blot,Simple Western,Immunocytochemistry |
| 目标/特异性 | Detects human EGF R/ErbB1 with the aa 746-750 deletion in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Simple Western: 20 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1956 (Human); 13649 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human EGF R/ErbB1 synthetic peptide CPVAIKTSPKAN Accession # P00533 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Epidermal growth factor receptor (EGF R, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. EGFR exon 19 deletions are in-frame deletions occurring within exon 19, which encodes part of the kinase domain. This mutation occurs with a frequency of approximately 48% in EGFR mutant lung tumors. It affects the catalytic domain (amino acids 746-750), and is predominantly associated with non-small cell lung cancer (1). In a metastatic setting, EGFR deletions like aa746-750 are predictors of efficacy of the EGFR tyrosine kinase inhibitors (1,2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human EGF R/ErbB1 (aa 746-750 deletion) by Western Blot. Western blot shows lysates of HCC827 human non-small cell lung cancer cell line, HCT‑116 human colorectal carcinoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EGF R/ErbB1 (aa 746-750 deletion) Monoclonal Antibody (Catalog # MAB8336) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EGF R/ErbB1 (aa 746-750 deletion) at approximately 170 kDa (as indicated). For additional reference total EGF R/ErbB1 was detected using Goat Anti-Human EGF R/ErbB1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF231). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
EGF R/ErbB1 (aa 746-750 deletion) in HCC827 Human Cell Line. EGF R/ErbB1 (aa 746-750 deletion) was detected in immersion fixed HCC827 human non-small cell lung cancer cell line using Mouse Anti-Human EGF R/ErbB1 (aa 746-750 deletion) Monoclonal Antibody (Catalog # MAB8336) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. Negative staining shown in the lower panel with NCI-H1975 human lung epithelial cell line. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human EGF R/ErbB1 by Simple WesternTM. Simple Western lane view shows lysates of HCC827 human non-small cell lung cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for EGF R/ErbB1 at approximately 200 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human EGF R/ErbB1 (aa 746-750 deletion) Monoclonal Antibody (Catalog # MAB8336). This experiment was conducted under reducing conditions and using the66-440 kDa separation system. |
