| 货号 | MAB3178-SP |
| 别名 | CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor |
| 反应种属 | Mouse |
| 应用 | Western Blot |
| 目标/特异性 | Detects mouse Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2152 (Human); 14066 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III Ala29-Glu251 Accession # P20352 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 - 28) and the mature chain (residues 29 - 294). As a type I membrane protein, it contains a transmembrane region (residues 252 - 274) and a cytoplasmic tail (residues 275 - 294) (3, 4). The purified recombinant mouse TF corresponds to the ectodomain (residues 29 - 251) and is potent in activating thermolysin-processed recombinant mouse Coagulation Factor VII (R&D Systems, Catalog # 3305‑SE) under the conditions described in the Activity Assay Protocol. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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