Human Coagulation Factor III/Tissue Factor MAb (Cl 323519) (25 UG)【科瑞奥博】

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Human Coagulation Factor III/Tissue Factor MAb (Cl 323519) (25 UG)

货号： MAB23391-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB23391-SP
别名	CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
反应种属	Human
应用	Western Blot,Flow Cytometry,CyTOF-ready
目标/特异性	Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2152 (Human); 14066 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice Authors: Q Zhang, H Wang, H Li, J Xu, K Tian, J Yang, Z Lu, J Zheng Oncotarget, 2017;8(6):9488-9499. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III Gly34-Glu251 Accession # P13726
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor, with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. Scarpati, E.M. et al. (1987) Biochemistry 26:5234. Fisher, K.L. et al. (1987) Thromb. Res. 48:89. Morrissey, J.H. et al. (1987) Cell 50:129. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

参考图片

Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB23391) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.