Human Coagulation Factor III/Tissue Factor MAb (Cl 323514) (25 UG)【科瑞奥博】

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Human Coagulation Factor III/Tissue Factor MAb (Cl 323514) (25 UG)

货号： MAB2339-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2339-SP
别名	CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
反应种属	Human
应用	Western Blot,Immunoprecipitation,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human coagulation factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Tissue Factor is observed.
使用方法	Western Blot: 2 µg/mL Immunoprecipitation: 25 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2152 (Human); 14066 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. Authors: Conroy AL, Liles WC, Molyneux ME PLoS ONE, 2011;6(12):e28540. Species: Human Sample Type: Plasma Application: ELISA Development Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL. Authors: Mendez-Cruz AR, Paez A, Jimenez-Flores R, Reyes-Reali J, Varela E, Cerbulo-Vazquez A, Rodriguez E, Lopez-Marure R, Masso FA, Flores-Romo L, Montano LF Immunol. Lett., 2007;111(2):116-23. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III Gly34-Glu251 Accession # P13726
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor, with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. Scarpati, E.M. et al. (1987) Biochemistry 26:5234. Fisher, K.L. et al. (1987) Thromb. Res. 48:89. Morrissey, J.H. et al. (1987) Cell 50:129. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

参考图片

Detection of Human Coagulation Factor III by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line treated (+) with TNF-alpha. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB2339) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Coagulation Factor III at approximately 50 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.