| 货号 | MAB9081-SP |
| 别名 | CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL | 全称 | Matrix Metalloproteinase 8 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry,CyTOF-ready,Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects both pro and active forms of human MMP-8 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP-1, -2, -3, -7, -9, -10, -12, or -13 is observed. |
| 使用方法 | Immunohistochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4317 (Human); 17394 (Mouse); 63849 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Matrix metalloproteinase-8 is expressed in human chorion during labor. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human MMP‑8 Phe21-Gly467 Accession # AAZ38714 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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MMP‑8 in Human Breast Cancer Tissue. MMP‑8 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 8 µg/mL Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB9081) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in terminal ductules (round) and intralobular duct (elongated) composing glandular lobules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
