Human Fas Ligand/TNFSF6 MAb (Clone 100419) (25 UG)【科瑞奥博】

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Human Fas Ligand/TNFSF6 MAb (Clone 100419) (25 UG)

货号： MAB126-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB126-SP
别名	apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6
反应种属	Human
应用	ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human Fas Ligand/TNFSF6 in ELISAs.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Fas Ligand/TNFSF6-induced cytotoxicity in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND₅₀) is typically 1-5 ng/mL in the presence of 2 ng/mL Recombinant Human Fas Ligand/TNFSF6 and 10 µg/mL of a cross-linking antibody, Mouse poly-Histidine Monoclonal Antibody.
来源	Monoclonal Mouse IgG_2B Clone # 100419
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	356 (Human); 14103 (Mouse); 25385 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice. Authors: Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W PLoS ONE, 2015;10(8):e0135999. Species: Human Sample Type: Whole Cells Application: Neut Potent anti-tumor killing activity of the multifunctional Treg cell line HOZOT against human tumors with diverse origins. Authors: Inoue T, Tashiro Y, Takeuchi M Int. J. Oncol., 2011;38(5):1299-306. Species: Human Sample Type: Whole Cells Application: Neut KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis. Authors: Navenot JM, Fujii N, Peiper SC Mol. Pharmacol., 2009;75(5):1074-83. Species: Human Sample Type: Whole Cells Application: Neut Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells. Authors: Krysov SV, Krysov</LastName><ForeNam SV</Initia, Rowley TF, Al-Shamkhani A Mol. Cancer Ther., 2007;6(2):703-11. Species: Human Sample Type: Whole Cells Application: Neut A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK Cell Death Differ., 2006;13(10):1789-801. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human Fas Ligand/TNFSF6 Pro134-Leu281 Accession # P48023
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain (2). Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Both mouse and human Fas Ligand are active on mouse and human cells (2, 3). Fas Ligand is expressed on the cell surface as a nondisulfide-linked homotrimer on activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta, however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684. Takahashi, T. et al. (1994) Int. Immunol. 6:1567. Seino, K-I. et al. (1998) J. Immunol. 161:4484. Suda, T. et al. (1993) Cell 75:1169. Pitti, R.M. et al. (1998) Nature 396:699. Schneider, P. et al. (1998) J. Exp. Med. 187:1205. Tanaka, M. et al. (1998) Nature Med. 4:31. Chen, J-J. et al. (1998) Science 282:1714. Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. USA 97:1707. Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228. Ryan, A.E. et al. (2005) Cancer Res. 65:9817. Takahashi, T. et al. (1994) Cell 76:969. Lynch, D.H. et al. (1994) Immunity 1:131.

参考图片

Cytotoxicity Induced by Fas Ligand/TNFSF6 and Neutralization by Human Fas Ligand/TNFSF6 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050), Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induces cytotoxicity in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human Fas Ligand/TNFSF6 (2 ng/mL) is neutralized (green line) by increasing concentrations of Human Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB126). The ND₅₀ is typically 1-5 ng/mL.