Human Ubiquitin MAb (Clone 1002A) (25 UL)【科瑞奥博】

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Human Ubiquitin MAb (Clone 1002A) (25 UL)

货号： MAB8595-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB8595-SP
简介	Recombinant Monoclonal Antibody
别名	RPS27A; UBA52; UBB ubiquitin B; UBB; UBC
反应种属	Human
应用	Western Blot
目标/特异性	Detects human Ubiquitin in direct ELISAs
使用方法	Western Blot: 1:1000 dilution
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7314 (Human); 298693 (Rat)
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	HEK293 human embryonic kidney cell line transfected with human Ubiquitin Accession # P0CG47
生物活性	Human
标记	Unconjugated
背景	Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell Structure and Signaling Molecules Cell to Cell Adhesion Disassembly During EMT CXCR4: A Receptor for Extracellular Ubiquitin HIF Transcription Factors Negative Regulators of the Jak/STAT Pathway NFkB Pathway Notch Pathway Parkinsons Disease Related Molecules Ubiquitin Ubiquitin-related Molecules in the Akt Pathway Wnt Intracellular Signaling

参考图片

Detection of Human Ubiquitin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with MG132. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Ubiquitin Monoclonal Antibody
(Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Ubiquitin at various molecular weights (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Ubiquitin by Western Blot. Western blot shows Di-Ubiquitin chains of the indicated linkage type loaded at 2 ng/lane. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Di-Ubiquitin as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.