Human/Mouse SSEA-4 MAb (Cl MC-813-70) (25 UG)【科瑞奥博】

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Human/Mouse SSEA-4 MAb (Cl MC-813-70) (25 UG)

货号： MAB1435-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1435-SP
别名	SSEA4; SSEA-4
全称	Stage-specific Embryonic Antigen-4
反应种属	Human/Mouse
应用	Flow Cytometry,Immunocytochemistry
目标/特异性	Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan PLoS ONE, 2016;11(4):e0152993. Species: Human Sample Type: Whole Cells Application: IHC Fresh Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors. Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S PLoS ONE, 2014;9(1):e84547. Species: Human Sample Type: Whole Cells Application: ICC Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells. Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen Sci Rep, 2014;4(0):3779. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells. Authors: Kim SY, Kim MJ, Jung H Stem Cells Dev., 2012;21(8):1272-86. Species: Human Sample Type: Whole Cells Application: ICC Human dermis harbors distinct mesenchymal stromal cell subsets. Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A J. Invest. Dermatol., 2012;132(3):563-74. Species: Human Sample Type: Whole Tissue Application: IHC Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells. BMC Vet Res, 2012;8(0):150. Species: Canine Sample Type: Whole Cells Application: Flow Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji PLoS ONE, 2012;7(9):e45435. Species: Human Sample Type: Whole Cells Application: ICC Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Authors: Emdad L, DSouza SL, Kothari HP, Qadeer ZA, Germano IM Stem Cells Dev., 2012;21(3):404-10. Species: Human Sample Type: Whole Cells Application: Flow Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells. Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS Mol. Ther., 2011;19(3):584-93. Species: Human Sample Type: Whole Cells Application: ICC A bioinformatic assay for pluripotency in human cells. Authors: Muller FJ, Schuldt BM, Williams R, Mason D, Altun G, Papapetrou EP, Danner S, Goldmann JE, Herbst A, Schmidt NO, Aldenhoff JB, Laurent LC, Loring JF Nat. Methods, 2011;8(4):315-7. Species: Human Sample Type: Whole Cells Application: ICC Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH FEBS Lett., 2009;583(3):561-7. Species: Human Sample Type: Whole Cells Application: ICC Tumor initiating potential of side population cells in human gastric cancer. Authors: Fukuda K, Saikawa Y, Ohashi M, Kumagai K, Kitajima M, Okano H, Matsuzaki Y, Kitagawa Y Int. J. Oncol., 2009;34(5):1201-7. Species: Human Sample Type: Whole Cells Application: ICC Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types. Authors: Reed SA, Johnson SE J. Cell. Physiol., 2008;215(2):329-36. Species: Equine Sample Type: Whole Cells Application: ICC Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells. Authors: Fong H, Hohenstein KA, Donovan PJ Stem Cells, 2008;26(8):1931-8. Species: Human Sample Type: Whole Cells Application: ICC Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ Stem Cells, 2008;26(6):1436-43. Species: Human Sample Type: Whole Cells Application: ICC Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Stem Cells, 2007;25(2):490-9. Species: Human Sample Type: Whole Cells Application: Flow Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Authors: Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM Stem Cells, 2007;25(2):437-46. Species: Human Sample Type: Whole Cells Application: Flow Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH FEBS Lett., 2006;580(25):5869-74. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	2120Ep human embryonal carcinoma cell line
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).
运输条件	Blue Ice
存放说明	4℃
参考文献	Shevinsky, L.H. et al. (1982) Cell 30:697. Kannagi, R. et al. (1983) EMBO J. 2:2355. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53. Draper, J.S. et al. (2002) J. Anat. 200:249.

参考图片

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

SSEA‑4 in BG01V Human Stem Cells. SSEA‑4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA‑4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.