Mouse IL-18 R alpha/IL-1 R5 MAb (Clone 112624) (25 UG)【科瑞奥博】

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Mouse IL-18 R alpha/IL-1 R5 MAb (Clone 112624) (25 UG)

货号： MAB12161-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB12161-SP
别名	CD218a; IL-1 R5; IL-1 Rrp; IL-18 R1; IL18R1
全称	Interleukin 18 Receptor alpha
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL‑1 RII, rhIL‑1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in activated mouse T cells. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑18/IL‑1F4 and 0.1 ng/mL Recombinant Mouse IL‑12.
来源	Monoclonal Rat IgG_2A Clone # 112624
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8809 (Human); 16174 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria. Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N J. Immunol., 2011;187(3):1333-46. Species: Mouse Sample Type: In Vivo Application: In vivo Protection of CD8+ T cells from activation-induced cell death by IL-18. Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H J. Leukoc. Biol., 2007;82(1):142-51. Species: Mouse Sample Type: Whole Cells Application: Neut IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation. Authors: Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA J. Immunol., 2007;178(3):1948-59. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha Ser19-Gly326 Accession # Q61098
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Angiogenesis gamma delta T Cells Human CD Marker Products ICC / IHC Images: IL-18 R alpha/IL-1 R5 IL-1 Family Inflammasomes: Intracellular Regulators of Pathogen Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells Mini-review: Interleukin 18 (IL-18) Neuroinflammation T Cell Cytokine Signaling Th1 Cells

参考图片

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND₅₀ is typically 0.2-0.8 µg/mL.