Human Serpin E1/PAI-1 MAb (Clone 1121C) (25 UL)【科瑞奥博】

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Human Serpin E1/PAI-1 MAb (Clone 1121C) (25 UL)

货号： MAB17861-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB17861-SP
简介	Recombinant Monoclonal Antibody
别名	Endothelial plasminogen activator inhibitor; Nexin; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1
全称	Plasminogen Activator Inhibitor
反应种属	Human
应用	Western Blot,Simple Western
目标/特异性	Detects human Serpin E1/PAI‑1 in direct ELISAs.
使用方法	Western Blot: 1:1000 dilution Simple Western: 1:100 dilution
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5054 (Human); 18787 (Mouse)
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin E1/PAI‑1 Met1-Pro402 Accession # NP_000593
生物活性	Human
标记	Unconjugated
背景	As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI‑1 is the principle inhibitor of urokinase‑type plasminogen activator (uPA) and tissue‑type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI‑1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N‑terminal signal peptide (6, 7). PAI‑1 may exist in one of two possible conformations, designated as active or latent (8). The purified rhPAI‑1 is active against rhuPA. The heterogeneity at the N‑terminus of the purified rhPAI‑1 has been observed before for both the recombinant and native proteins (9).
运输条件	Blue Ice
存放说明	4℃
参考文献	Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293. Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545. Duffy, M.J. (2002) Clin. Chem. 48:1194. Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196. Pannekoek, H. et al. (1986) EMBO J. 5:2539. Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673. Wang, Z. et al. (1996) Biochemistry 35:16443. Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309.

参考图片

Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB17861) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Serpin E1/PAI‑1 by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 55 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB17861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.