| 货号 | MAB711-SP |
| 别名 | colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim | 全称 | Granulocyte Macrophage Growth Factor |
| 反应种属 | Porcine |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects porcine GM-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots (reducing conditions), approximately 20% cross‑reactivity with recombinant rat GM-CSF is observed and less than 1% cross-reactivity with recombinant human GM-CSF and recombinant mouse GM-CSF is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 40 ng/mL Recombinant Porcine GM‑CSF. |
| 来源 | Monoclonal Mouse IgG1 Clone # 111802 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant porcine GM-CSF Ala18-Lys144 Accession # Q29118 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8‑10). Mature porcine GM-CSF shares 61%‑72% amino acid sequence identity with canine, feline, human, and rat GM-CSF and 53% with mouse GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse (14). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Cell Proliferation Induced by GM‑CSF and Neutralization by Porcine GM‑CSF Antibody. Recombinant Porcine GM‑CSF (Catalog # 711-PG) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM‑CSF (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine GM‑CSF Monoclonal Antibody (Catalog # MAB711). The ND50 is typically 1-4 µg/mL. |
