Human MMP-7 MAb (Clone 111433) (25 UG)【科瑞奥博】

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Human MMP-7 MAb (Clone 111433) (25 UG)

货号： MAB9071-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB9071-SP
别名	EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase
全称	Matrix Metalloproteinase 7
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunoprecipitation
目标/特异性	Detects the pro and active forms of human MMP-7 in Western blots. In dot blots, approximately 20% cross-reactivity with recombinant human MMP-8 is observed and no cross-reactivity with recombinant human MMP-1, -2, -3, -9, -10, -12, or -13 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4316 (Human); 17393 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer. Authors: Szarvas T, Singer BB, Becker M, vom Dorp F, Jager T, Szendroi A, Riesz P, Romics I, Rubben H, Ergun S BJU Int., 2011;107(7):1069-73. Species: Human Sample Type: Urine Application: IP Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces. Authors: Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B J. Cell. Sci., 2011;124(0):369-83. Species: Human Sample Type: Whole Cells Application: Flow Matrix metalloproteinase-7 as a marker of metastasis and predictor of poor survival in bladder cancer. Authors: Szarvas T, Becker M, vom Dorp F, Gethmann C, Totsch M, Bankfalvi A, Schmid KW, Romics I, Rubben H, Ergun S Cancer Sci., 2010;101(5):1300-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Matrix metalloproteinase activity in pediatric acute lung injury. Authors: Kong MY, Gaggar A, Li Y Int J Med Sci, 2009;6(1):9-17. Species: Human Sample Type: Endotracheal aspirate Application: WB Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery. Authors: Yu KH, Barry CG, Austin D J. Proteome Res., 2009;8(3):1565-76. Species: Human Sample Type: Serum Application: WB Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model. Authors: Gogly B, Fournier B, Couty L, Naveau A, Brasselet C, Durand E, Coulomb B, Lafont A J. Mol. Cell. Cardiol., 2009;47(2):296-303. Species: Rabbit Sample Type: Cell Culture Supernates Application: WB Divergent functions for airway epithelial matrix metalloproteinase 7 and retinoic acid in experimental asthma. Authors: Goswami S, Angkasekwinai P, Shan M, Greenlee KJ, Barranco WT, Polikepahad S, Seryshev A, Song LZ, Redding D, Singh B, Sur S, Woodruff P, Dong C, Corry DB, Kheradmand F Nat. Immunol., 2009;10(5):496-503. Species: Human Sample Type: Whole Tissue Application: IHC IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G J. Immunol., 2007;178(9):5957-65. Species: Human Sample Type: Cell Culture Supernates Application: WB
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP‑7 Leu18-Lys267 (Ala230del) Accession # NP_002414.1
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha ‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell to Cell Adhesion Disassembly During EMT Collagen Modifying Enzymes ICC / IHC Images: MMP-7 Inflammatory Mediators Metalloproteases and Regulators MMP Modulation of Cytokines MMP-12 in Smoking-Related Pulmonary Disease MMPs, TIMPs and Related Molecules New Tools: Active MMP Activity Assays OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression

参考图片

Detection of Human MMP‑7 by Western Blot. Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP‑7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.