Human Caspase-3 MAb (Clone 1112I) ( )
货号：
MAB7071-SP 基本售价：
1467.3 元 规格：
-
产品信息
概述| 货号 | MAB7071-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA |
| 应用 | Immunocytochemistry |
| 目标/特异性 | Detects human Caspase-3 in direct ELISAs. |
| 使用方法 | Immunocytochemistry: 1-25 µg/mL
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 836 (Human); 12367 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Human Caspase-3 synthetic peptide
Accession # P42574 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Apoptosis: Caspase Pathways
- Apoptosis: Mitochondria Accused of Murder
- Caspases and Regulators
- Cell Structure and Signaling Molecules
- Cysteine Proteases and Regulators
- ICC / IHC Images: Caspase-3
- Interactive Pathway: Apoptosis Signaling Pathways
- Mini-review: Caspases
- Novel Caspase-8 Roles Revealed by Knockouts
- Technical Notes: Caspase Antibodies
- TNF Superfamily Regulators
|
参考图片 Caspase‑3 in Jurkat Human Cell Line. Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine using Rabbit Anti-Human Caspase‑3 Monoclonal Antibody (Catalog # MAB7071) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
