Mouse CCL17/TARC MAb (Clone 110904) (25 UG)【科瑞奥博】

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Mouse CCL17/TARC MAb (Clone 110904) (25 UG)

货号： MAB529-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB529-SP
别名	ABCD-2; CC chemokine TARC; C-C motif chemokine 17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
反应种属	Mouse
应用	ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse CCL17/TARC in ELISAs.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND₅₀) is typically 0.75-3.0 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL17/TARC.
来源	Monoclonal Rat IgG_2A Clone # 110904
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6361 (Human); 20295 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation J Clin Invest, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo Engagement of two distinct binding domains on CCL17 is required for signaling through CCR4 and establishment of localized inflammatory conditions in the lung. Authors: Santulli-Marotto S, Boakye K, Lacy E, Wu S, Luongo J, Kavalkovich K, Coelho A, Hogaboam C, Ryan M PLoS ONE, 2013;8(12):e81465. Species: Mouse Sample Type: recombinant protein Application: DirELISA Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development CCL17 controls mast cells for the defense against filarial larval entry. Authors: Specht S, Frank JK, Alferink J, Dubben B, Layland LE, Denece G, Bain O, Forster I, Kirschning CJ, Martin C, Hoerauf A J. Immunol., 2011;186(8):4845-52. Species: Mouse Sample Type: In Vivo Application: In vivo Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM Am. J. Respir. Crit. Care Med., 2009;179(9):772-81. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17. Authors: Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM J. Immunol., 2008;181(12):8576-84. Species: Mouse Sample Type: Whole Cells Application: Neut CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. Authors: Kearley J, Robinson DS, Lloyd CM J. Allergy Clin. Immunol., 2008;122(3):617-24.e6. Species: Mouse Sample Type: BALF Application: ELISA Development The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Authors: Fujita H, Asahina A, Komine M, Tamaki K Cell. Immunol., 2007;245(2):70-9. Species: Mouse Sample Type: Whole Cells Application: Neut iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity. Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT J. Immunol., 2007;179(7):4661-71. Species: Mouse Sample Type: Whole Cells Application: Neut 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA Blood, 2004;104(5):1404-10. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived Recombinant Mouse CCL17/TARC Ala24-Pro93 Accession # Q9WUZ6
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Imai, T. et al. (1997) J. Biol. Chem. 272:15036. Imai, T. et al. (1996) J. Biol. Chem. 271:21514. Nomiyama, H. et al. (1997) Genomics 40:211. Lieberam, I.et al. (1999) Eur. J. Immunol. 29:2684.

参考图片

Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody. Recombinant Mouse CCL17/TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL17/TARC Monoclonal Antibody (Catalog # MAB529). The ND₅₀ is typically 0.75-3.0 µg/mL.