| 货号 | MAB8571-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CCXCR1; CCXCR1XC chemokine receptor 1; chemokine (C motif) receptor 1; chemokine XC receptor 1; G protein-coupled receptor 5; GPR5; GPR5chemokine (C motif) XC receptor 1; G-protein coupled receptor 5; Lymphotactin receptor | 全称 | Chemokine [C Motif] Receptor 1 |
| 反应种属 | Human |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human XCR1 in direct ELISAs. |
| 使用方法 | Flow Cytometry: 0.25 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2829 (Human); 23832 (Mouse); 301086 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant human XCR1 Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267 Accession # P46094 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS |
| 背景 | XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of XCR1 in CD141+ and HLA-DR+ Human Blood Monocytes by Flow Cytometry.CD141+ and HLA-DR+ human peripheral blood monocytes were isolated by staining with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated MonoclonalAntibody (Catalog # FAB4869F)and Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog #FAB3947A). Isolated cells were then stained with (B) RabbitAnti-Human XCR1 Monoclonal Antibody (Catalog # MAB8571, filled histogram) orisotype control antibody (Catalog # AB-105-C,open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG SecondaryAntibody (Catalog # F0110). |
