Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets.
Authors: Micklem D, Blo M, Bergstrom P, Hodneland E, Tiron C, Hoiby T, Gjerdrum C, Hammarsten O, Lorens J
BMC Biotechnol, 2014;14(0):57.
Species: Human
Sample Type: Whole Cells
Application: Flow
Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
PLoS Pathog, 2014;10(12):e1004548.
Species: Human
Sample Type: Whole Cells
Application: Flow
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
J. Exp. Med., 2012;209(11):2033-47.
Species: Human
Sample Type: Whole Cells
Application: Flow
Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry.
Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y
J. Virol., 2012;86(4):2067-78.
Species: Human
Sample Type: Whole Cells
Application: Flow
Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
Authors: Zhu D, Wang Y, Singh I
Blood, 2010;115(23):4963-72.
Species: Human
Sample Type: Cell Lysates
Application: WB
Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions.
Authors: Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B
Am. J. Pathol., 2009;175(1):283-93.
Species: Human
Sample Type: Whole Tissue
Application: IHC Frozen
Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
J. Immunol., 2009;183(5):3004-13.
Species: Human
Sample Type: Whole Cells
Application: cell separation
Multiple roles for the receptor tyrosine kinase axl in tumor formation.
Authors: Holland SJ, Powell MJ, Franci C, Chan EW, Friera AM, Atchison RE, McLaughlin J, Swift SE, Pali ES, Yam G, Wong S, Lasaga J, Shen MR, Yu S, Xu W, Hitoshi Y, Bogenberger J, Nör JE, Payan DG, Lorens JB
Cancer Res., 2005;65(20):9294-303.
Species: Human
Sample Type: Cell Lysates
Application: WB

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.