A2B5 MAb (Clone 105) (25 UG)【科瑞奥博】

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A2B5 MAb (Clone 105) (25 UG)

货号： MAB1416-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1416-SP
别名	A2B5
应用	Flow Cytometry,CyTOF-ready,Immunocytochemistry
目标/特异性	Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis. Authors: Robinson A, Rodgers J, Goings G, Miller S PLoS ONE, 2015;9(9):e107649. Species: Mouse Sample Type: Whole Cells Application: Flow Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration. Authors: Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C J Neurosci, 2015;35(1):4-20. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen In vivo generation of neural tumors from neoplastic pluripotent stem cells models early human pediatric brain tumor formation. Authors: Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M Stem Cells, 2012;30(3):392-404. Species: Human Sample Type: Whole Cells Application: Flow Disrupted-in-Schizophrenia-1 expression is regulated by beta-site amyloid precursor protein cleaving enzyme-1-neuregulin cascade. Authors: Seshadri S, Kamiya A, Yokota Y, Prikulis I, Kano S, Hayashi-Takagi A, Stanco A, Eom TY, Rao S, Ishizuka K, Wong P, Korth C, Anton ES, Sawa A Proc. Natl. Acad. Sci. U.S.A., 2010;107(12):5622-7. Species: Human Sample Type: Whole Cells Application: ICC Musashi1 RNA-binding protein regulates oligodendrocyte lineage cell differentiation and survival. Authors: Dobson NR, Zhou YX, Flint NC, Armstrong RC Glia, 2008;56(3):318-30. Species: Rat Sample Type: Whole Cells Application: ICC Tim-2 is the receptor for H-ferritin on oligodendrocytes. Authors: Todorich B, Zhang X, Slagle-Webb B, Seaman WE, Connor JR J. Neurochem., 2008;107(6):1495-505. Species: Rat Sample Type: Whole Cells Application: ICC AMPA-mediated excitotoxicity in oligodendrocytes: role for Na(+)-K(+)-Cl(-) co-transport and reversal of Na(+)/Ca(2+) exchanger. Authors: Chen H, Kintner DB, Jones M, Matsuda T, Baba A, Kiedrowski L, Sun D J. Neurochem., 2007;102(6):1783-95. Species: Rat Sample Type: Whole Cells Application: ICC Cyclopamine treatment of human embryonic stem cells followed by culture in human astrocyte medium promotes differentiation into nestin- and GFAP-expressing astrocytic lineage. Authors: Lee DS, Yu K, Rho JY, Lee E, Han JS, Koo DB, Cho YS, Lee KK, Han YM Life Sci., 2006;80(2):154-9. Species: Human Sample Type: Whole Cells Application: ICC Efficient induction of oligodendrocytes from human embryonic stem cells. Authors: Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW Stem Cells, 2006;25(2):419-24. Species: Human Sample Type: Whole Cells Application: ICC Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms. Authors: Rosin C, Bates TE, Skaper SD J. Neurochem., 2004;90(5):1173-85. Species: Human Sample Type: Whole Cells Application: ICC High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK Brain Res. Dev. Brain Res., 2004;152(2):159-69. Species: Human Sample Type: Whole Cells Application: ICC Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal. Authors: Tsai HH, Tessier-Lavigne M, Miller RH Development, 2003;130(10):2095-105. Species: Chicken Sample Type: Whole Cells Application: ICC
纯化方式	IgM-specific Affinity-purified from hybridoma culture supernatant
免疫原	Embryonic chicken retinal cells
生物活性	Human, Mouse, Rat, Chicken
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).
运输条件	Blue Ice
存放说明	4℃
参考文献	Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

参考图片

Detection of A2B5 in RAW 264.7 Mouse Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

A2B5 in NTera-2 Human Cell Line. A2B5 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days) using Mouse Anti-Human/Mouse/Rat/
Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.