| 货号 | MAB2091-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | Cytokine P40; HP40; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40 | 全称 | Interleukin 9 |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human IL-9 in Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3578 (Human); 16198 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9 Gln19-Ile144 Accession # P15248 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Interleukin-9 (IL-9) is a heavily glycosylated cytokine with a 14 kDa peptide backbone that is secreted by CD4+ Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human IL‑9 by Western Blot. Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), and Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # 202-IL), Recombinant Human IL‑4 (Catalog # 204-IL), Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF‑ beta 1 (Catalog # 240-B). PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human IL‑9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL‑9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
