| 货号 | MAB15841-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | signal transducer and activator of transcription 5B; STAT5; transcription factor STAT5B | 全称 | Signal Transducer and Activator of Transcription 5b |
| 反应种属 | Human |
| 应用 | Western Blot,Simple Western,Immunocytochemistry,Knockout Validated |
| 目标/特异性 | Detects human, mouse, and rat STAT5b in Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution Simple Western: 1:100 dilution Immunocytochemistry: 1:100 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6777 (Human); 20851 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Human STAT5b synthetic peptide aa 777-787 Accession # P51692 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 背景 | Signal Transduction and Activator of Transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL‑22, IL-6, IFN-alpha ). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation, and survival. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat STAT5b by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitts lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, M1 mouse myeloid leukemia cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5b at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
STAT5b in HepG2 Human Cell Line. STAT5b was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841) at a 1:100 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human and Mouse STAT5b by Simple WesternTM. Simple Western lane view shows lysates of M1 mouse myeloid leukemia cell line and Daudi human Burkitts lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5b at approximately 90 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
