| 货号 | MAB1171-SP |
| 别名 | IL-12A | 全称 | Interleukin 12 p35 |
| 反应种属 | Porcine |
| 应用 | Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects the heterodimeric form of porcine IL‑12 (p35/p40) in direct ELISAs. Western blotting data indicates recognition of the IL-12/IL-35 p35 subunit. In direct ELISAs and Western blots, 20‑100% cross-reactivity with the disulfide‑linked heterodomeric forms of recombinant mouse IL‑12 and recombinant human IL-12 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3592 (Human); 16159 (Mouse); 84405 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p35/p40 heterodimer p40 Ile23-Asn324, p35 Arg26-Ser222 Accession # Q28938, Q29053 |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Porcine IL-12 subunits p35 and p40 share about 85% homology to the human subunits, but differ by containing a 3 aa addition in the p35 subunit and a 4 aa deletion in the p40 subunit. Porcine IL-12 induces proliferation of human lymphoblasts and IFN-gamma secretion by human and porcine lymph nodes. Porcine IL-12 has been detected in lymphoid tissues including inguinal and mesenteric lymph nodes, Peyers patches, spleen and thymus. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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