| 货号 | MAB7721-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | 5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor | 全称 | Extracellular Matrix Metalloproteinase Inducer |
| 反应种属 | Mouse |
| 应用 | Western Blot,Immunohistochemistry |
| 目标/特异性 | Detects mouse EMMPRIN/CD147 in direct ELISAs. |
| 使用方法 | Western Blot: 1:1000 dilution Immunohistochemistry: 1:200-1:400 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 682 (Human); 12215 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with mouse EMMPRIN/CD147 Ala22-Pro209 Accession # P18572 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 背景 | EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse EMMPRIN/CD147 by Western Blot. Western blot shows lysates of mouse brain tissue and mouse heart tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EMMPRIN/CD147 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
EMMPRIN/CD147 in Mouse Testis. EMMPRIN/CD147 was detected in perfusion fixed frozen sections of mouse testis using Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) at a 1:250 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to spermatozoa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
