Human HMGB1 MAb (Clone 115603) (25 UG)【科瑞奥博】

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Human HMGB1 MAb (Clone 115603) (25 UG)

货号： MAB1690-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1690-SP
别名	Amphoterin; high mobility group box 1; High mobility group protein 1; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG1DKFZp686A04236; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
全称	High Mobility Group Protein 1
反应种属	Human
应用	Western Blot,Chromatin Immunoprecipitation (ChIP),CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human HMGB1/HMG-1 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.05 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3146 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology. Authors: Moser B, Janik S, Schiefer A, Mullauer L, Bekos C, Scharrer A, Mildner M, Renyi-Vamos F, Klepetko W, Ankersmit H PLoS ONE, 2014;9(4):e94118. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Measles virus causes immunogenic cell death in human melanoma. Authors: Donnelly, O G, Errington-Mais, F, Steele, L, Hadac, E, Jennings, V, Scott, K, Peach, H, Phillips, R M, Bond, J, Pandha, H, Harrington, K, Vile, R, Russell, S, Selby, P, Melcher, A A Gene Ther, 2013;20(1):7-15. Species: Human Sample Type: cell culture supernate Application: WB Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis. Authors: Liou T, Adler F, Keogh R, Li Y, Jensen J, Walsh W, Packer K, Clark T, Carveth H, Chen J, Rogers S, Lane C, Moore J, Sturrock A, Paine R, Cox D, Hoidal J PLoS ONE, 2012;7(8):e42748. Species: Human Sample Type: Sputum Application: ELISA Development Human Papillomavirus Type 8 Interferes with a Novel C/EBPÃŽÂ²-Mediated Mechanism of Keratinocyte CCL20 Chemokine Expression and Langerhans Cell Migration. Authors: Sperling T, Oldak M, Walch-Ruckheim B, Wickenhauser C, Doorbar J, Pfister H, Malejczyk M, Majewski S, Keates AC, Smola S PLoS Pathog., 2012;8(7):e1002833. Species: Human Sample Type: Cell Lysates Application: WB Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease. Authors: Vitali R, Stronati L, Negroni A Am. J. Gastroenterol., 2011;106(11):2029-40. Species: Human Sample Type: Feces Application: WB Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection. Authors: Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ J. Hepatol., 2009;51(2):322-32. Species: Human Sample Type: Cell Lysates Application: WB Ethyl pyruvate decreases HMGB1 release and ameliorates murine colitis. Authors: Dave SH, Tilstra JS, Matsuoka K, Li F, DeMarco RA, Beer-Stolz D, Sepulveda AR, Fink MP, Lotze MT, Plevy SE J. Leukoc. Biol., 2009;86(3):633-43. Species: Mouse Sample Type: Feces Application: ELISA Development Potential role of high-mobility group box 1 in cystic fibrosis airway disease. Authors: Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, ONeal W, Sorscher EJ, Abraham E, Blalock JE Am. J. Respir. Crit. Care Med., 2008;178(8):822-31. Species: Human Sample Type: Saliva Application: WB E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release. Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP Int. J. Cancer, 2008;123(8):1741-50. Species: Human Sample Type: Cell Culture Supernates Application: Immunodepletion TLR4 enhances TGF-beta signaling and hepatic fibrosis. Authors: Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF Nat. Med., 2007;13(11):1324-32. Species: Mouse Sample Type: Cell Lysates Application: WB Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalate. Authors: Zetterstrom CK, Jiang W, Wahamaa H, Ostberg T, Aveberger AC, Schierbeck H, Lotze MT, Andersson U, Pisetsky DS, Erlandsson Harris H J. Leukoc. Biol., 2007;83(1):31-8. Species: Mouse Sample Type: Cell Culture Supernates Application: WB IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. Authors: Rao DA, Tracey KJ, Pober JS J. Immunol., 2007;179(10):6536-46. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human HMGB1/HMG-1 Gly2-Glu215 (predicted) Accession # P09429
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1‑3). Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu (4, 5). Residues 27‑43 and 178‑184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues (6). HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Lotze, M.T. and K.J. Tracey (2005) Nat. Rev. Immunol. 5:331. Yang, H. et al. (2005) J. Leukoc. Biol. 78:1. Dumitriu, I.E. et al. (2005) Trends Immunol. 26:381. Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11. Wen, L. et al. (1989) Nucleic Acids Res. 17:1197. Bonaldi, T. et al. (2003) EMBO J. 22:5551. Muller, S. et al. (2001) EMBO J. 20:4337. Bustin, M. (1999) Mol. Cell. Biol. 19:5237. Wang, H. et al. (1999) Science. 285:248. Dimitriu, I.E. et al. (2005) J. Immunol. 174:7506. Najima, Y. et al. (2005) J. Biol. Chem. 280:27523. Gardella, S. et al. (2002) EMBO Rep. 3:995.

参考图片

Detection of Human HMGB1/HMG‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human HMGB1/HMG‑1 Monoclonal Antibody (Catalog # MAB1690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HMGB1/HMG‑1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of HMGB1/HMG‑1-regulated Genes by Chromatin Immunoprecipitation. HepG2 human hepatocellular carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HMGB1/HMG‑1/DNA complexes were immunoprecipitated using 5 μg Mouse Anti-Human HMGB1/HMG‑1 PE-conjugated Monoclonal Antibody (Catalog # MAB1690) or control antibody (Catalog # MAB004) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The AFP promoter was detected by standard PCR.

Detection of HMGB1 in HCT‑116 Human Cell Line by Flow Cytometry. HCT‑116 human colorectal carcinoma cell line was stained with Mouse Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB1690, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B).

HMGB1/HMG‑1 in CCD‑18Co Human Cell Line. HMGB1/HMG‑1 was detected in immersion fixed CCD‑18Co human colonic fibroblast cell line using Mouse Anti-Human HMGB1/HMG‑1 PE-conjugated Monoclonal Antibody (Catalog # MAB1690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.