Human Decorin MAb (Clone 115402) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Decorin MAb (Clone 115402) (25 UG)

货号： MAB143-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB143-SP
别名	Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B
反应种属	Human
应用	Western Blot,Immunohistochemistry
目标/特异性	Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse Decorin.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1634 (Human); 13179 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Proteomics of thyroid tumours provides new insights into their molecular composition and changes associated with malignancy Authors: J Martínez-A, R Clifton-Bl, MP Molloy Sci Rep, 2016;6(0):23660. Species: Human Sample Type: tissue lysates Application: WB Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta. Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F J Bone Miner Res, 2015;30(8):1445-56. Species: Human Sample Type: Cell Culture Supernates Application: WB Possible dual role of decorin in abdominal aortic aneurysm. Authors: Ueda, Koshiro, Yoshimura, Koichi, Yamashita, Osamu, Harada, Takasuke, Morikage, Noriyasu, Hamano, Kimikazu PLoS ONE, 2015;10(3):e0120689. Species: Human Sample Type: Tissue Homogenates Application: WB Faulty initiation of proteoglycan synthesis causes cardiac and joint defects. Authors: Baasanjav S, Al-Gazali L, Hashiguchi T, Mizumoto S, Fischer B, Horn D, Seelow D, Ali BR, Aziz SA, Langer R, Saleh AA, Becker C, Nurnberg G, Cantagrel V, Gleeson JG, Gomez D, Michel JB, Stricker S, Lindner TH, Nurnberg P, Sugahara K, Mundlos S, Hoffmann K Am. J. Hum. Genet., 2011;89(1):15-27. Species: Human Sample Type: Cell Culture Supernates Application: WB Lack of CXC chemokine receptor 3 signaling leads to hypertrophic and hypercellular scarring. Authors: Yates CC, Krishna P, Whaley D, Bodnar R, Turner T, Wells A Am. J. Pathol., 2010;176(4):1743-55. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Role of decorin in the antimyeloma effects of osteoblasts. Authors: Li X, Pennisi A, Yaccoby S Blood, 2008;112(1):159-68. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin Gly17-Lys172 Accession # NP_598013.1
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.
运输条件	Blue Ice
存放说明	4℃
参考文献	Naito, Z. (2005) J. Nippon Med. Sch. 72:137. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771. Danielson, K. et al. (1993) Genomics 15:146.

参考图片

Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.