| 货号 | MAB780-SP |
| 别名 | DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 | 全称 | Interleukin 1 Receptor Antagonist |
| 反应种属 | Porcine |
| 应用 | Western Blot |
| 目标/特异性 | Detects porcine IL‑1ra/IL‑1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse IL-1ra/IL-1F3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant porcine IL‑1ra/IL‑1F3 His26-Gln177 Accession # Q29056 |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptors (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitroandin vivo. Human, mouse and rat IL-1ra all share approximately 80% homology with porcine IL-1ra. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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