| 货号 | MAB8634-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; PREF1; Pref-1; protein delta homolog 1; secredeltin; ZOG | 全称 | Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot,Simple Western,Flow Cytometry,Immunohistochemistry |
| 目标/特异性 | Detects mouse Pref-1 in direct ELISAs. |
| 使用方法 | Western Blot: 1:1000 dilution Simple Western: 1:100 dilution Flow Cytometry: 4 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8788 (Human); 13386 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1 Ala24-Gln305 Accession # Q09163 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 背景 | Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385). It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and mouse embryo tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Pref‑1/DLK1/FA1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). | |
Detection of Mouse Pref‑1/DLK1/FA1 by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for Pref‑1/DLK1/FA1 at approximately 63 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
