| 货号 | MAB89661-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | FHL3; HLH3; HPLH3; Munc13-4; Munc13-4protein unc-13 homolog D; unc-13 homolog D (C. elegans) | 全称 | UNC-13 Homolog D |
| 反应种属 | Human |
| 应用 | Western Blot,Simple Western,Immunocytochemistry |
| 目标/特异性 | Detects the conventional form of human UNC13D in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Simple Western: 1 µg/mL Immunocytochemistry: 5-25 µg/mL |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 201294 (Human); 70450 (Mouse); 192177 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | KLH-coupled N-terminal human UNC13D peptide Accession # Q70J99 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS |
| 背景 | UNC13D (also Munc13-4) is a 123 kDa cytoplasmic andperipheral membrane protein that is expressed at highest levels in hematopoietictissues. UNC13D appears to play a role in vesicle maturation duringexocytosis and its expression is obligatory for exocytosis of cytolyticgranules from NK and T cells. A point mutation in intron 1 of UNC13D causesfamilial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessiveimmune deficiency. The mutation disrupts transcription factor binding andprevents expression of an alternative isoform that is required for lymphocytecytotoxicity. The conventional and alternative isoforms are identical fromamino acids (aa) 40-1090 but have distinct N-terminal segments. Human and mouse have 75% aa sequence identity throughout the region used as the immunogen. It is unknown whether mice express the alternative isoform. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human UNC13D by Western Blot. Western blot shows lysates of NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and Ramos human Burkitts lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for UNC13D at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
UNC13D in NK‑92 Human Cell Line. UNC13D was detected in immersion fixed NK‑92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
Detection of Human UNC13D by Simple WesternTM. Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB89661). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
