| 货号 | MAB7761-SP |
| 别名 | IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6 | 全称 | Insulin-like Growth Factor Binding Protein 6 |
| 反应种属 | Mouse |
| 应用 | Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects mouse IGFBP-6 in ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant human (rh) IGFBP-3 and rhIGFBP-5 is observed and no cross-reactivity with rhIGFBP-1, recombinant mouse (rm) IGFBP-1, rhIGFBP-2, rmIGFBP-2, rmIGFBP-3, rhIGFBP-4, rmIGFBP-5, or rhIGFBP-6 is observed. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3489 (Human); 16012 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-6 Gly24-Gly238 Accession # P47880 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-6 cDNA encodes a 238 amino acid (aa) precursor protein with a putative 25 aa signal peptide that is processed to generate the 213 aa mature protein that is O-glycosylated. Mouse and human IGFBP-6 share 73% aa similarity. Mouse and rat IGFBP-6 share 94% aa similarity and the mouse IGFBP-6 has a 9 amino acid insertion compared to the rat homolog. IGFBP-6 is expressed in ovarian, testicular, muscle, heart and lung tissues in the adult mouse. IGFBP-6 was not detected in total RNA from a whole mouse embryo. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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