Streptavidin MAb (Clone 1220C) (25 UG)
货号：
MAB9020-SP 基本售价：
1467.3 元 规格：
-
产品信息
概述| 货号 | MAB9020-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | SA V1; SA V2; Streptavidin V1; Streptavidin V2 |
| 应用 | Western Blot |
| 目标/特异性 | Detects Streptavidin in Western blot. |
| 使用方法 | Western Blot: 0.5 µg/mL
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Purified streptavidin from Streptomyces avidinii |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Streptavidin is a 52.8 kDa protein from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetramer forming a beta-barrel with an extraordinarily high affinity for biotin (also known as vitamin B7). It has a dissociation constant (Kd) on the order of ≈10−14mol/L.The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature and has a high resistance to organic solvents, denaturants, detergents, proteolytic enzymes, and extreme pH ranges or temperatures. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
参考图片 Detection of Streptavidin by Western Blot. Western blot shows recombinant Streptavidin. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Streptavidin Monoclonal Antibody (Catalog # MAB9020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Streptavidin at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
