货号 | MAB27591-SP |
简介 | Recombinant Monoclonal Antibody |
别名 | ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; TOLL; toll-like receptor 4 | 全称 | Toll-like Receptor 4 |
应用 | Flow Cytometry,Immunocytochemistry |
目标/特异性 | Detects mouse TLR4 in direct ELISAs. |
使用方法 | Flow Cytometry: 0.25 µg/106cells Immunocytochemistry: 1-25 µg/mL |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 7099 (Human); 21898 (Mouse); 29260 (Rat) |
纯化方式 | Protein A or G purified from cell culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TLR4 Asn26-Lys629 Accession # Q9QUK6 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0110). | |
TLR4 in RAW 264.7 Mouse Cell Line. TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog #NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |