| 货号 | MAB8594-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CMH7; TNNC1; TNNI3 |
| 反应种属 | Human |
| 应用 | Western Blot,Simple Western,Immunocytochemistry |
| 目标/特异性 | Detects human Troponin I in direct ELISAs. |
| 使用方法 | Western Blot: 1:1000 dilution Simple Western: 1:100 dilution Immunocytochemistry: 1:100 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7137 (Human); 21954 (Mouse); 29248 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Peptide 3 Accession # P19429 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat Troponin I by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Troponin I at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Troponin I in Human Cardiomyocytes. Troponin I was detected in immersion fixed human embryonic stem cells, differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032), using Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594) at 1:100 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006). Cardiac Troponin T was also detected in cardiomyocytes using Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (Catalog # MAB1874). Cells were co-stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining of Troponin I was localized to cardiac muscle fibrils. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human Troponin I by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.1 mg/mL. A specific band was detected for Troponin I at approximately 25-35 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
