| 货号 | MAB827-SP |
| 别名 | apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2 | 全称 | B Cell Lymphoma/Leukemia 2 |
| 反应种属 | Human |
| 应用 | Western Blot,Simple Western |
| 目标/特异性 | Detects human Bcl-2 in Western blots. |
| 使用方法 | Western Blot: 0.1-0.5 µg/mL Simple Western: 5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 596 (Human); 12043 (Mouse); 24224 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mediation of electronegative low-density lipoprotein signaling by LOX-1: a possible mechanism of endothelial apoptosis. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Bcl-2 minus the carboxyl-terminal mitochondrial targeting sequence Met1-Asp211 Accession # P10415 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
|
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Bcl‑2 Minus C-Terminus by Western Blot. Western blot shows lysates of KG‑1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.1-0.5 µg/mL of Mouse Anti-Human Bcl‑2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB827) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl‑2 Minus C-Terminus at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Bcl‑2 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for Bcl‑2 at approximately 24 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Bcl‑2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB827). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
