| 货号 | MAB4532-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CINC-1; GRO alpha; KC; MGSA-alpha |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse CXCL1/GRO alpha /KC/CINC‑1 in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 1-2 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2919 (Human); 14825 (Mouse); 81503 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC‑1 Asn29-Lys96 Accession # P12850 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 背景 | KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet‑derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulant for CFU-GM. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CXCL1/GRO alpha /KC/CINC‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) treated with 1 μg/mL LPS and Brefeldin A for 4 hours or (B) untreated were stained with Rabbit Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB4532) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Rat Anti-Mouse TNF‑ alpha Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). |
