Human/Mouse XIAP MAb (Clone 117320) (25 UG)【科瑞奥博】

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Human/Mouse XIAP MAb (Clone 117320) (25 UG)

货号： MAB822-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB822-SP
别名	API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; X-linked IAP; X-linked inhibitor of apoptosis
全称	X-linked Inhibitor of Apoptosis
反应种属	Human/Mouse
应用	Western Blot
目标/特异性	Detects human and mouse XIAP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rhcIAP-2 is observed.
使用方法	Western Blot: 2 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	331 (Human); 11798 (Mouse); 63879 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Protein kinase C zeta regulates survivin expression and inhibits apoptosis in colon cancer. Authors: Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N Int J Oncol, 2014;45(3):1043-50. Species: Human Sample Type: Total protein Application: WB Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals. Authors: Schoenmann Z, Assa-Kunik E, Tiomny S, Minis A, Haklai-Topper L, Arama E, Yaron A J. Neurosci., 2010;30(18):6375-86. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human XIAP Met1-Ser497 Accession # P98170
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	XIAP (X-chromosome linked inhibitor of apoptosis) is a member of theapoptosis (IAP) family of proteins that inhibit caspases. The BIR2domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP toinhibit caspases is prevented by SMAC/Diablo through binding toXIAP-BIR2 and -BIR3 domains.
运输条件	Blue Ice
存放说明	4℃
参考文献	Caspases and Regulators Cysteine Proteases and Regulators ICC / IHC Images: XIAP Inhibitors of Apoptosis (IAPs) and Regulators

参考图片

Detection of Human/Mouse XIAP by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibrosarcoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human XIAP Full Length (Catalog # 822-XF) and recombinant mouse XIAP (5 ng/lane) were included. A specific band for XIAP was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.