| 货号 | MAB8648-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CASP11; Caspl; Ich-3 |
| 反应种属 | Mouse |
| 应用 | Western Blot |
| 目标/特异性 | Detects mouse Caspase-11/Caspase-4 in Western blots. |
| 使用方法 | Western Blot: 1:1000 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 12363 (Mouse); 114555 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | HEK293 human embryonic kidney cell line transfected with mouse Caspase-11/Caspase-4 Accession # P70343 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 背景 | Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines. Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen. The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373). Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines. Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS. The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta. Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Caspase-11/Caspase-4 by Western Blot. Western blot shows lysates of mouse splenocytes untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB8648) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase-11/Caspase-4 at approximately 36, 44, and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
