| 货号 | MAB8375-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | BEND8; BTB (POZ) domain containing 14B; BTB/POZ domain-containing protein 14B; BTBD14B; BTBD30; NAC1BEN domain containing 8; NAC-1FLJ37383; NACC1; nucleus accumbens associated 1, BEN and BTB (POZ) domain containing; nucleus accumbens-associated protein 1; transcriptional repressor NAC1 | 全称 | Nucleus Accumbens Associated 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot,Simple Western,Immunohistochemistry |
| 目标/特异性 | Detects mouse NAC1 in direct ELISAs. |
| 使用方法 | Western Blot: 1:1000 dilution Simple Western: 1:100 dilution Immunohistochemistry: 1:70-1:100 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 112939 (Human); 66830 (Mouse); 171454 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse NAC1 Val457-Gln514 Accession # Q7TS78 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 背景 | NAC1 (Nucleus Accumbens-associated Protein 1) also known as NACC1, is a 527 amino acid (aa) BTB/POZ domain containing protein. Human NAC1 shares 86% and 85% aa sequence identity with mouse and rat NAC1, respectively. It has been shown to have a role in transcriptional regulation in both pluripotent stem cells and in the brain. Additionally, NAC1 expression is upregulated in many cancers and this expression has been suggested to correlate with increased aggressiveness in some cancers. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse NAC1 by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, F9 mouse teratocarcinoma stem cells, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NAC1 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
NAC1 in Mouse Embryo. NAC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) at a 1:80 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to developing primary sensory neurons in dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Mouse NAC1 by Simple WesternTM. Simple Western lane view shows lysate of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for NAC1 at approximately 69 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
