Mouse CCL2/JE/MCP-1 MAb (Clone 123616) (25 UG)【科瑞奥博】

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Mouse CCL2/JE/MCP-1 MAb (Clone 123616) (25 UG)

货号： MAB479-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB479-SP
别名	MCAF; MCP-1
反应种属	Mouse
应用	Neutralization
目标/特异性	Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human(rh) CCL1, 2, 3,4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,rmCCL1, 3, 4, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12,17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.
使用方法	Neutralization: Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 0.75-3.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.
来源	Monoclonal Rat IgG_2B Clone # 123616
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor Nat Med, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut Near-infrared laser adjuvant for influenza vaccine. Authors: Kashiwagi, Satoshi, Yuan, Jianping, Forbes, Benjamin, Hibert, Mathew L, Lee, Eugene L, Whicher, Laura, Goudie, Calum, Yang, Yuan, Chen, Tao, Edelblute, Beth, Collette, Brian, Edington, Laurel, Trussler, James, Nezivar, Jean, Leblanc, Pierre, Bronson, Roderick, Tsukada, Kosuke, Suematsu, Makoto, Dover, Jeffrey, Brauns, Timothy, Gelfand, Jeffrey, Poznansky, Mark C PLoS ONE, 2013;8(12):e82899. Species: Mouse Sample Type: tissue Application: IHC Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1. Authors: Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, Cheng XW, Egashira K, Matsushita T, Nakamura H, Murohara T Diabetes, 2012;61(6):1552-61. Species: Mouse Sample Type: In Vivo Application: In Vivo Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung. J. Immunol., 2012;189(5):2545-52. Species: Mouse Sample Type: Whole Cells Application: Neut Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization. Authors: Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS J. Biol. Chem., 2011;286(23):20991-1001. Species: Mouse Sample Type: In Vivo Application: In vivo Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice. Authors: Venet F, Huang X, Chung CS, Chen Y, Ayala A Am. J. Pathol., 2010;176(2):764-73. Species: Mouse Sample Type: In Vivo Application: In vivo MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis. Authors: Narasaraju T, Ng HH, Phoon MC Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43. Species: Mouse Sample Type: In Vivo Application: In vivo The role for monocyte chemoattractant protein-1 in the generation and function of memory CD8+ T cells. Authors: Wang T, Dai H, Wan N, Moore Y, Dai Z J. Immunol., 2008;180(5):2886-93. Species: Mouse Sample Type: In Vivo Application: In vivo Upregulation of VEGF in murine retina via monocyte recruitment after retinal scatter laser photocoagulation. Authors: Itaya M, Sakurai E, Nozaki M, Yamada K, Yamasaki S, Asai K, Ogura Y Invest. Ophthalmol. Vis. Sci., 2007;48(12):5677-83. Species: Mouse Sample Type: In Vivo Application: In vivo CCL2 regulates angiogenesis via activation of Ets-1 transcription factor. Authors: Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV J. Immunol., 2006;177(4):2651-61. Species: Mouse Sample Type: In Vivo Application: In vivo Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during inflammation induced by lipopolysaccharide or Mycobacterium bovis bacille Calmette-Guerin. Authors: Penido C, Vieira-de-Abreu A, Bozza MT, Castro-Faria-Neto HC, Bozza PT J. Immunol., 2003;171(12):6788-94. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CCL2/JE/MCP-1 Gln24-Arg96 Accession # P10148
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Rollins, B.J.et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738. Gu, L.et al. (1999) Chem. Immunol. 72:7. Luini, W.et al. (1994) Cytokine 6:28.

参考图片

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND₅₀ is typically 0.75-3.0 µg/mL.