Human MMP-14/MT1-MMP MAb (Clone 128527) (25 UG)【科瑞奥博】

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Human MMP-14/MT1-MMP MAb (Clone 128527) (25 UG)

货号： MAB9181-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB9181-SP
别名	EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1
全称	Matrix Metalloproteinase 14/Membrane Type 1 MMP
反应种属	Human
应用	Western Blot,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,CyTOF-ready
目标/特异性	Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4323 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Contractile forces contribute to increased glycosylphosphatidylinositol-anchored receptor CD24-facilitated cancer cell invasion. Authors: Mierke CT, Bretz N, Altevogt P J. Biol. Chem., 2011;286(40):34858-71. Species: Human Sample Type: Whole Cells Application: Flow Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces. Authors: Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B J. Cell. Sci., 2011;124(0):369-83. Species: Human Sample Type: Whole Cells Application: Flow GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing. Authors: Djafarzadeh R, Noessner E, Engelmann H Oncogene, 2006;25(10):1496-508. Species: Human Sample Type: Cell Culture Supernates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP Tyr112-Ala541 (predicted) Accession # P50281
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha _V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail (2).
运输条件	Blue Ice
存放说明	4℃
参考文献	Seike, M. (2003) Cancer Lett. 194:1. Sato, H. et al. (1994) Nature 370:61.

参考图片

Detection of MMP-14/MT1-MMP in
MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).