Human MCAM/CD146 MAb (Clone 128018) (25 UG)【科瑞奥博】

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Human MCAM/CD146 MAb (Clone 128018) (25 UG)

货号： MAB932-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB932-SP
别名	CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin
全称	Melanoma Cell Adhesion Molecule
反应种属	Human
应用	Western Blot,Flow Cytometry,CyTOF-ready,Immunocytochemistry
目标/特异性	Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1 is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4162 (Human); 84004 (Mouse); 78967 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor. Authors: Im, Hyungsoo, Shao, Huilin, Park, Yong Il, Peterson, Vanessa, Castro, Cesar M, Weissleder, Ralph, Lee, Hakho Nat Biotechnol, 2014;32(5):490-5. Species: N/A Sample Type: Cell Lysates Application: Antibody Profiling
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human MCAM Val24-Gly559 Accession # AAA20824
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	MCAM, also known as MUC18, is a putative adhesion molecule that belongsto the immunoglobulin superfamily. It is associated with tumorprogression and metastasis of various carcinomas and may be involved inembryonic neural development.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipose-derived Stem Cell Markers Cancer Biomarkers Dendritic Cell Adhesion and Migration Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Endothelial Progenitor and Endothelial Cell Markers Endothelial Progenitor Cell Markers Human CD Marker Products ICC / IHC Images: MCAM/CD146 Immunoglobulin Superfamily CAMs Myogenesis Markers Osteogenesis Markers Osteoprogenitor Markers Skeletal Muscle Markers Special Glycoproteins

参考图片

Detection of Human MCAM/CD146 by Western Blot. Western blot shows lysate of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCAM/CD146 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MCAM/CD146 in BG01V Human Embryonic Stem Cells. MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.