| 货号 | MAB846-SP |
| 别名 | apoptosis regulator BAX; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4 | 全称 | Bcl Associated X Protein |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects full-length human Bax in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 581 (Human); 12028 (Mouse); 24887 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The role of nerve growth factor in hyperosmolar stress induced apoptosis. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Bax aa 1-171 Accession # Q07812 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Bax belongs to the Bcl-2 protein family. It forms homodimers andheterodimers with Bcl-2 and functions as an apoptotic activator. Baxinteracts with the mitochondrial voltage dependent anion channel andpromotes the release of cytochrome c. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human Bax Minus C-Terminus by Western Blot. Western blot shows lysate of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |
