| 货号 | MAB222-SP |
| 别名 | activin A receptor, type IB; Activin receptor type IB; Activin receptor-like kinase 4; ACTRIB; ACVR1B; ACVRLK4ACTR-IB; ALK-4; ALK4activin A receptor, type II-like kinase 4; EC 2.7.11; EC 2.7.11.30; Serine/threonine-protein kinase receptor R2; SKR2activin receptor type-1B | 全称 | Activin Receptor IB |
| 反应种属 | Human |
| 应用 | Western Blot,Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects human Activin RIB/ALK-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Activin RIA, rhActivin RIIA, or rhActivin RIIB. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.2-1 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Cripto 1 (Catalog # 145-CR) to immobilized Recombinant Human Activin RIB/ALK-4 Fc Chimera (Catalog # 808-AR) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Mouse IgG1 Clone # 126822 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 91 (Human); 11479 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Activin RIB/ALK-4 Ser24-Glu126 Accession # AAA60556 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25-35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7-9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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