货号 | MAB340-SP |
别名 | activin A receptor, type II; activin A receptor, type IIA; Activin receptor type IIA; activin receptor type-2A; ACTRII; ACTRIIA; ACVR2A; ACVR2ACTR-IIA; AVR2A; EC 2.7.11; EC 2.7.11.30 | 全称 | Activin Receptor IIA |
反应种属 | Human |
应用 | Western Blot |
目标/特异性 | Detects human Activin RIIA in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Activin RIA, rhActivin RIB, or rhActivin RIIB. |
使用方法 | Western Blot: 1 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 92 (Human) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIIA Ala20-Pro134 Accession # P27037 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human Activin RIIA by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Activin RIIA Monoclonal Antibody (Catalog # MAB340) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Activin RIIA at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |