Human CD14 MAb (Clone 134603) (25 UG)【科瑞奥博】

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Human CD14 MAb (Clone 134603) (25 UG)

货号： MAB3831-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB3831-SP
别名	CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
反应种属	Human
应用	Western Blot
目标/特异性	Detects human CD14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD14 is observed.
使用方法	Western Blot: 5 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. 1,25-Dihydroxyvitamin D3 promotes a sustained LPS-induced NF-kappaB-dependent expression of CD55 in human monocytic THP-1 cells. Authors: Izban M, Nowicki B, Nowicki S PLoS ONE, 2012;7(11):e49318. Species: Human Sample Type: Cell Lysates Application: WB Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells. Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M Clin. Exp. Immunol., 2009;156(2):285-93. Species: Human Sample Type: Saliva Application: Immunodepletion Soluble CD14 and toll-like receptor-2 are potential salivary biomarkers for oral lichen planus and burning mouth syndrome. Authors: Srinivasan M, Kodumudi KN, Zunt SL Clin. Immunol., 2007;126(1):31-7. Species: Human Sample Type: Saliva Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human CD14 Thr20-Cys352 Accession # P08571
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) precursor protein with a 19 aa signal peptide and a C‑terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.
运输条件	Blue Ice
存放说明	4℃
参考文献	Wright, S.D.et al. (1990) Science 249:1431. Pugin, J.et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744. Beutler, B. (2000) Current Opinion in Immunology 12:20. Stelter, F. (2000) Chem. Immunol. 74:25.

参考图片

Detection of Human CD14 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 5 µg/mL Human CD14 Monoclonal Antibody (Catalog # MAB3831) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human CD14 (Catalog # 383-CD) (5 ng) was included. A specific band for CD14 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.