Mouse CCL28 MAb (Clone 134306) (25 UG)【科瑞奥博】

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Mouse CCL28 MAb (Clone 134306) (25 UG)

货号： MAB533-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB533-SP
别名	CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse CCL28 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) CCL28 is observed and no cross-reactivity with rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16,17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7,9/10, 11, 12, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL28-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND₅₀) is typically 2.5-5.0 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL28.
来源	Monoclonal Rat IgG_2B Clone # 134306
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	56477 (Human); 56838 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Structure-function analysis of CCL28 in the development of post-viral asthma. Authors: Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B J Biol Chem, 2015;290(7):4528-36. Species: Mouse Sample Type: In Vivo Application: In vivo Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. Authors: Cha HR, Ko HJ, Kim ED J. Immunol., 2011;187(6):3044-52. Species: Mouse Sample Type: In Vivo Application: In vivo Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Nature, 2011;475(7355):226-30. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo. Authors: Kutzler MA, Kraynyak KA, Nagle SJ, Parkinson RM, Zharikova D, Chattergoon M, Maguire H, Muthumani K, Ugen K, Weiner DB Gene Ther., 2010;17(1):72-82. Species: Mouse Sample Type: Cell Lysates Application: IP Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development. Authors: Mosconi E, Rekima A, Seitz-Polski B Mucosal Immunol, 2010;3(5):461-74. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CCL28 Ile23-Arg130 Accession # Q9JIL2
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.
运输条件	Blue Ice
存放说明	4℃
参考文献	Wang, W.et al. (2000) J. Biol. Chem. 275:22313.

参考图片

Chemotaxis Induced by CCL28 and Neutralization by Mouse CCL28 Antibody. Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL28 Monoclonal Antibody (Catalog # MAB533). The ND₅₀ is typically 2.5-5.0 µg/mL.