货号 | MAB134-SP |
别名 | WIF-1; WNT inhibitory factor 1 | 全称 | Wnt Inhibitory Factor |
反应种属 | Human |
应用 | Western Blot,Immunohistochemistry |
目标/特异性 | Detects human WIF-1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse WIF‑1 is observed. |
使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 11197 (Human); 24117 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Evidence for altered Wnt signaling in psoriatic skin. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human WIF-1 Gly29-Trp379 Accession # AAD25402 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | WIF-1 is a secreted protein that binds Wnt proteins and antagonizesWnt-induced signaling. WIF-1 expression is down regulated in severalhuman cancers. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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WIF‑1 in Human Breast. WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |